Abstract: Glycome refers to the collection of glycans produced by cell and tissue under defined situations of time, location and environment. Synthesis of glycan on protein is strictly regulated by genes while rarely affected by the environment. Besides, Serum glycome is very stable at the individual level. In the studies of liver diseases, researchers have found significant changes in fucosylation, brancing, bisecting N-acetylglucosamine and sialic acid of glycans. This article reviews the advances in the application of glycome test in the diagnosis and treatment of liver fibrosis, liver cirrhosis, liver cancer and liver transplantation.