Abstract: Cardiovascular toxicity induced by anticancer treatment seriously affects the prognosis of patients. The clinical manifestations of cardiovascular toxicity varys with different anticancer treatments, but its mechanisms are still poorly understood. With in-depth researches in epigenetics, it has been found that many diseases are closely related to nucleic acid methylation. With the purpose of providing new clues and thoughts for preventing cardiovascular toxicity of oncotherapy, this review focuses on the roles of nucleic acid methylation in cardiovascular toxicity of oncotherapy.