Abstract: Ischemic stroke (IS) is a central nervous system disease with complex pathological mechanisms.Currently, there is no perfect strategy for rapid differential diagnosis or absolutely safe and effective comprehensive therapeutic method for IS, which poses a huge threat to human health.Traditional Chinese medicine (TCM) has the characteristics of multi-component, multi-target and multi-pathway treatment, with its unique advantages in comprehensive treatment of IS.In recent years, metabolomics has developed rapidly, providing a new tool for IS diagnosis based on biomarkers and a new technical platform for the study of TCM treatment of IS.This study reviewed the application of metabolomics in diagnosis and treatment of IS with traditional Chinese medicines, in order to provide reference for further development of the diagnosis and treatment system of IS.