Abstract: RNA therapy, which regulates gene expression by introducing exogenous RNAs into specific cells, is a very promising strategy for disease treatment. However, the delivery of RNA drugs require suitable drug delivery systems, because RNAs are unstable in vivo and cannot enter target cells efficiently. Compared with viral vectors, non-viral vectors are safer, and have become a research hotspot. In this article, we mainly introduced three non-viral delivery systems that may enable the clinical translation of RNA therapeutics:lipid nanoparticle delivery systems, bioconjugation delivery systems, and exosome delivery systems. The related technical progress and difficulties were also analyzed and summarized.