Abstract: Microneedles, as an emerging and efficient delivery system in transdermal drug delivery, can selectively penetrate the stratum corneum to overcome the limitations of drug absorption. They have such advantages as minimal invasiveness, painlessness, high compliance, and controlled release, with broad prospects in the field of biomedical transdermal drug delivery. This review briefly summarizes the development and research progress of microneedles in recent years, systematically discusses the advantages and disadvantages of various types of microneedles in medical application, and particularly introduces the research progress of microneedle preparation technology in the biomedical field, in order to provide some reference for the further development and application of microneedles.