Abstract: Adipose-derived stromal vascular fraction(SVF) cells are a heterogeneous cell population with the characteristics of stem cells. Because of their various cell types, they have strong regenerative potentials such as angiogenesis, tissue remodeling and immune regulation. They have been used for clinic treatment of various conditions with good safety and therapeutic effects. This article reviewed the different separation methods of adipose-derived SVF and characterizes the specific phenotypes of the cells contained in it. At the same time, the advances in clinical applications of SVF in fat transplantation, diabetes, Crohn's disease, osteoarthritis and other diseases were summarized.