Abstract: In recent years, electrochemical analysis has become a technique widely used in pharmaceutical analysis, involving many fields such as drug quality control, drug metabolism research and toxic substance detection. It is inseparable from the advent and development of a large number of new electrochemical sensors. As modification material of electrode sensors, chemical and biological functional materials can better exert the advantages of electrochemical analytical techniques like facile operation, quick response, high sensitivity, low detection limit and good selectivity, which can help researchers complete in situ, online, real-time, dynamic and complex analytical tasks to obtain more physiological and biochemical information. In this paper, the application of electrochemical transducers in pharmaceutical electroanalytical chemistry is reviewed from four aspects:inorganic nanomaterials, molecularly imprinted polymer, enzymes and DNA, with some brief comments on the future development of pharmaceutical electroanalytical chemistry.